Next generation sequencing analysis of cancer

Next-generation sequencing (NGS) study – This technology involve randomly sequencing the whole human genome in 70 to 100bp short fragments with 60 fold coverage. Each of these short fragments will be aligned back to the human genome sequence using the various NGS software tools (Samtools, MAQ, BWA etc) so as to generate sequence alignment, calling single nucleotide polymorphism, insertion or deletion compared with the reference human genome. The mutations detected will be compared with reference database to infer mutation information in the cancer samples. NGS study for one single sample will generate 750 GB of data to be mapped to the human genome. Our study intent to generation data for large number of cancer samples in comparison with normal so as to derive the somatic mutation landscape of cancer.